intracytoplasmic sperm injection (lcsi) in mice by a new holding pipette
نویسندگان
چکیده
mice are ideal models for icsi experiments because of the ease of culturing eggs/embryos in vitro and the availability of ample genetic information. unfortunately, it has been extremely difficult. in this study we improved the mouse icsi method by using a new holding pipette that was made of two pipettes such that one pipette was pulled and heat merged into the other one. the outer pipette had an outer diameter of 120 j..l. and an inner diameter of 80 j..l. to 85 j.1 (about mouse oocyte diameter). the inner pipette had an outer diameter of 80 µ to 85 µ. and an inner diameter of 55 µ to 60 µ. which was polished and narrowed to 20 j..l.on amicroforge. the distance between the tips of the two pipettes was adjusted to 120 µ. to 160 µ (1.5 to 2 fold longer than mouse oocyte diameter). of 307 oocytes which were injected with a single spermatozoon, 206 (67.1 %) survived and 93 (45.1 %) of surviving oocytes showed normal fertilization (2 pronuclei and second polar body). of 109 oocytes which were only sucked into the holding pipette (control group), 105 (96.3%) survived and only 4 (3.8%) of them became activated parthenogenically. u sing this new holding pipette, the oocyte is sucked into a glass tunnel and elongates to a reasonable length therefore the injection axis will be increased and piercing of the oolemma can be performed easily.
منابع مشابه
INTRACYTOPLASMIC SPERM INJECTION (lCSI) IN MICE BY A NEW HOLDING PIPETTE
Mice are ideal models for ICSI experiments because of the ease of culturing eggs/embryos in vitro and the availability of ample genetic information. Unfortunately, it has been extremely difficult. In this study we improved the mouse ICSI method by using a new holding pipette that was made of two pipettes such that one pipette was pulled and heat merged into the other one. The outer pipette ...
متن کاملNew Advances in Intracytoplasmic Sperm Injection (ICSI)
In these last twenty years, intracytoplasmic sperm injection (ICSI) has efficiently permitted the treatment of male factor infertility (Van Steirteghem et al., 1993); the direct injection of spermatozoa into ooplasm has allowed the embryologist to overcome low sperm motility, poor sperm-Zona Pellucida (ZP) binding, and defective acrosome reaction. Although ICSI has been successfully applied wor...
متن کاملIn vitro fertilization by intracytoplasmic sperm injection.
Intracytoplasmic sperm injection (ICSI) is the latest, and by far the most efficient, variant of micromanipulation-assisted fertilization, whereby a single spermatozoon is selected, aspirated into a microinjection needle and injected to the oocyte cytoplasm. The development of this technique is mainly linked to application in human assisted reproduction for which it enables fertilization with d...
متن کاملIntracytoplasmic Sperm Injection
Intracytoplasmic Sperm Injection (ICSI) is an assisted reproductive technique [4] (ART) initially developed by Dr. Gianpiero D. Palermo [5] in 1993 to treat male infertility [6]. It is most commonly used in conjunction with in vitro [7] fertilization [8] (IVF) or a less commonly used technique called zygote intrafallopian transfer [9] (ZIFT). In natural fertilization [8], the sperm [10] must pe...
متن کاملIntracytoplasmic sperm injection (ICSI)
IVF has become a well-established treatment for certain types of infertility, including long-standing infertility due to tubal disease, endometriosis, unexplained infertility or infertility involving a male factor. Soon it became clear that certain couples with severe male-factor infertility could not be helped by conventional IVF. Extremely low sperm counts, impaired motility and/or abnormal m...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
medical journal of islamic republic of iranجلد ۱۳، شماره ۳، صفحات ۲۱۹-۲۲۳
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023